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 Register for Nov. 18 Web Lecture with Andy Brooks, PhD NuGEN Ovation Pre-Amp Solutions Customer Feature: W. Nicholas Haining, B.M., B.Ch. Publications of the Month Asuragen Adds NuGEN Ovation® Systems to Gene Expression Profiling Menu for RNA Amplification and Target Preparation Attending the 67th Annual Meeting of the Japanese Cancer Association (JCA), October 28 - 30 in Nagoya? Ovation® Systems Family of RNA Amplification and Labeling Products Customer Spotlight Send us a link and a brief overview of your papers and posters enabled by NuGEN Ovation® Systems, and we'll consider your submission for an upcoming issue. |
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Don't Miss This Upcoming NuGEN Web Lecture Series Event! Linear cDNA amplification for archiving, distributing and analyzing large numbers of limiting clinical samples via QPCR. Featured Speaker: Andrew I. Brooks, PhD, Director of the BRTC at University of Medicine and Dentistry of New Jersey November 18, 8:30am PST/4:30pm GMT
Get Valuable Results from Every Valuable Sample With NuGEN Ovation® Pre-Amplification Solutions— Sensitive, Reproducible, Simple, Automatable. In order to address some of the important questions of biology, technologies allowing for the isolation of individual cell types have proliferated. Unfortunately, cell types of interest are often rare and the approaches used to isolate them yield very few cells given the amount of starting material available in typical biological studies. As a result, researchers have been forced to work with very small amounts of RNA for their expression studies. In addition, these samples require significant time and expense to generate and are often unique and difficult, if not impossible, to re-generate. NuGEN Ovation® technologies allow for the amplification of small amounts of RNA generated from these samples in order to perform more sensitive expression studies and, importantly, in order to generate enough stable, storable material (cDNA) for later use. Customer Feature W. Nicholas Haining, B.M., B.Ch. Assistant Professor of Pediatrics Department of Pediatric Oncology, Dana-Farber Cancer Institute Division of Pediatric Hematology/Oncology, Children's Hospital Harvard Medical School A central challenge in immunology is to understand the basis for protective T cell immunity to vaccines and pathogens. However current approaches to quantify the functional state of antigen-specific T cells often fail to correlate with protective immunity in vivo, especially in humans. In recent years, we1, 2 and others3, 4 p01528, 5 have sought to classify the differentiation of T cells in animal models and humans using genomic approaches. These studies have illustrated the marked and complex changes in gene expression profiles that occur during the development of memory T cells. Moreover, they show that alterations in expression profiles from antigen-specific T cells can distinguish T cells rendered dysfunctional by chronic viral infection in mice and in humans1, 2. This body of work points to an emerging concept in which genomic signatures of T cell differentiation can serve as molecular predictors of T cell function, and thus immunity. One of the major limitations of the genomic analysis of T cell immunity in humans, is the limiting number of antigen-specific T cells that can be obtained from peripheral blood samples. Here, we have found NuGen technologies to be crucial. We have generated hundreds of high-quality microarray datasets from starting populations as small as one thousand sorted T cells. We now depend on the NuGen WT-Ovation™ Pico system to routinely generate high-quality data. With precious one-of-a-kind samples from patients, the reliability and quality of the assay system has made it a core part of our lab's operation. 1. Wherry, E.J., E. J. et al. Molecular signature of CD8+ T cell exhaustion during chronic viral infection. Immunity 27, 670-684 (2007). 2. Haining, W.N. et al. Identification of an evolutionarily conserved transcriptional signature of CD8 memory differentiation that is shared by T and B cells. J Immunol 181, 1859-1868 (2008). 3. Appay, V. et al. Sensitive gene expression profiling of human T cell subsets reveals parallel post-thymic differentiation for CD4+ and CD8+ lineages. Journal of Immunology 179, 7406-7414 (2007). 4. Kaech, S.M., S. M., Hemby, S., Kersh, E. & Ahmed, R. Molecular and functional profiling of memory CD8 T cell differentiation. Cell 111, 837-851 (2002). 5. Luckey, C. et al. Memory T and memory B cells share a transcriptional program of self-renewal with long-term hematopoietic stem cells. Proc Natl Acad Sci U S A 103, 3304-3309 (2006). Publications of the Month
The Journal of Immunology, 2008, 181: 1859-1868. ABSTRACT The development of the various cells of the immune system, and their recruitment into a concerted immune response has been one of the most well studied areas of human biology. Despite the efforts of numerous scientists over decades of studies, some fundamental questions regarding how our immune system works remain. One of those questions regards the process by which lymphocytes become memory cells in response to antigen encounter. Because blood preparations contains a wide range of many cell types at various states of differentiation, questions involving responses of specific types of cells to discreet biological events have been difficult to pursue empirically. Haining et al. and colleagues at several prominent clinical research centers have combined approaches to isolate specific cell types of interest, the use of model systems allowing for the controlled presentation of antigens to cells in culture, and sensitive highly parallel gene expression platforms to investigate the manner in which CD4+, CD8+, and B cell lineages become memory cells. Because the researchers were able to utilize the very small amounts of total RNA available in these studies (10ng of total RNA), they were able to discover a specific transcriptional signature shared by both T and B cell lineages in response to Ag presentation. This result has important implications regarding not only the processes by which our immune system mobilizes against infection, but also how HIV disrupts this process, as this characteristic memory differentiation signature is profoundly disrupted by HIV infection. Gene expression analysis in pregnant women and their infants identifies unique fetal biomarkers that circulate in maternal blood Jill L. Maron, Kirby L. Johnson, Donna Slonim, Chao-Qiang Lai, Marco Ramoni, Gil Alterovitz, Zina Jarrah, Zinger Yang, and Diana W. Bianchi J Clin Invest. 2007 October 1; 117(10): 3007-3019. Published online 2007 September 20. doi: 10.1172/JCI29959. ABSTRACT The discovery of fetal mRNA transcripts in the maternal circulation holds great promise for noninvasive prenatal diagnosis. To identify potential fetal biomarkers, we studied whole blood and plasma gene transcripts that were common to 9 term pregnant women and their newborns but absent or reduced in the mothers postpartum. RNA was isolated from peripheral or umbilical blood and hybridized to gene expression arrays. Gene expression, paired Student's t test, and pathway analyses were performed. In whole blood, 157 gene transcripts met statistical significance. These fetal biomarkers included 27 developmental genes, 5 sensory perception genes, and 22 genes involved in neonatal physiology. Transcripts were predominantly expressed or restricted to the fetus, the embryo, or the neonate. Real-time RT-PCR amplification confirmed the presence of specific gene transcripts; SNP analysis demonstrated the presence of 3 fetal transcripts in maternal antepartum blood. Comparison of whole blood and plasma samples from the same pregnant woman suggested that placental genes are more easily detected in plasma. We conclude that fetal and placental mRNA circulates in the blood of pregnant women. Transcriptional analysis of maternal whole blood identifies a unique set of biologically diverse fetal genes and has a multitude of clinical applications. Asuragen Adds NuGEN Ovation® Systems to Gene Expression Profiling Menu for RNA Amplification and Target Preparation  The Pharmacogenomic Services Division of Asuragen, Inc. has added NuGEN Ovation® Systems to its menu of gene expression profiling services and ENCOMPASS™ Services for FFPE portfolio.
The NuGEN Ovation Systems family of RNA amplification and labeling products enable life scientists to conduct sensitive, robust, global gene expression profiling and novel signature discovery using a variety of challenging biological samples and gene expression platforms. Ovation Systems are designed for use with a broad range of sample types including formalin-fixed, paraffin-embedded (FFPE) tissues, whole blood, tissue biopsies, laser-captured micro-dissected cells (LCM), sorted cells and others. Asuragen will initially offer NuGEN RNA amplification and target preparation services for FFPE tissue and whole blood samples on the Affymetrix GeneChip® platform, using established workflows:
Attending the 67th Annual Meeting of the Japanese Cancer Association (JCA), October 28 - 30 in Nagoya?  Stop by the MediBIC booth at the 67th Annual Meeting of the Japanese Cancer Association and learn about NuGEN Solutions.
Ovation® Systems Family of RNA Amplification and Labeling Products  The NuGEN Ovation® Systems family of RNA amplification and labeling products enables sensitive, robust gene expression profiling and novel signature discovery using a variety of challenging biological samples such as FFPE, whole blood, LCM, fine needle aspirates, tissue biopsies, sorted cell, and more. The flexible modular products let you chose your preferred analytical platform; Affymetrix 3' or Exon, Agilent, or Illumina microarrays, and qPCR.
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