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Customer Feature
Publication of the Month NuGEN Publication List Upcoming Events WT-Ovation™ One-Direct Amplification System Technical Report and Webinar NuGEN User Guide Update WT-Ovation™ Exon Module Technical Report Have you been published recently? Send us a link and a brief overview of your papers and abstracts enabled by a NuGEN Ovation® or Applause™ System. We'll include them in our database of scientific publications and consider your submission for an upcoming issue of our monthly newsletter. |
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Customer Feature Asterand Expanding Service To Include Expression Profiling From FFPE Samples  Asterand plc, a certified service provider utilizing NuGEN's products, is a leading global supplier of high quality, well characterized human tissue and human tissue-based research services to drug discovery scientists. The company provides human tissues and services to accelerate the identification and validation of therapeutic targets and enhance the selection of drug candidates for its clients.
As interest has grown in linking the molecular basis of disease to the clinical outcomes of patients, Asterand's clients have been increasingly drawn to utilizing specimens from donors where the treatment and therapeutic outcomes are known. Asterand has an extensive biorepository of materials with clinical outcomes data. However, the majority of these samples are in an FFPE format which can impact the ability to recover intact RNA suitable for quantitative RT-PCR studies. Asterand had utilized several commercial kits for recovering intact RNA from these tissues with little success. In 2008, the company approached NuGEN® to incorporate its Whole Transcriptome WT-Ovation RNA amplification kit into its PhaseZERO® Gene Expression service platform. Asterand now utilizes the kit on its CustomMapping™ product line that offers clients a platform for exploring diseased and normal gene expression using quantitative RT-PCR and specimens from its biorepository. The kit has performed admirably within the lab personnel's hands. As Dr. Samir Alhasan, Director of Cell and Molecular Services at Asterand's Detroit facility notes "NuGEN's Ovation RNA amplification kit literally rescued a difficult FFPE project we performed for a client in 2008. The laser capture micro-dissection (LCM) of the client's FFPE tumor yielded very few cells for study. The Ovation kit allowed us to recover and amplify sufficient RNA to complete quantitative RT-PCR successfully." The success of this project led the company to incorporate the kits into its platform and seek NuGEN certified provider status. Publication of the Month
From the Departments of Biochemistry, Chemistry, and Pharmacology Vanderbilt Institute of Chemical Biology, Center in Molecular Toxicology, and Vanderbilt-Ingram Comprehensive Cancer Center, Vanderbilt University School of Medicine, Nashville, TN 37232-0146 Address correspondence to: Lawrence J. Marnett, Vanderbilt University School of Medicine, Nashville, TN 37232-0146 Summary Heat shock factor 1 (HSF1) promotes cytoprotective heat shock gene expression, and silencing HSF1 sensitizes cells to 4-Hydroxynonenal (HNE) induced apoptosis. The authors of this study used Affymetrix GeneChip 1.0 ST arrays to investigate the regulation of the Bcl-2 gene family by HSF1. This gene family is of interest due to the established roles of its members in an array of diseases including arterial restenosis, rheumatoid arthritis, hepatic carcinoma, multiple myeloma, prostate cancer, and colon cancer. In the gene expression portion of this study, HSF1 was silenced by siRNA transfection, and control and silenced RNA samples from normal colon and colon cancer cell lines were amplified in triplicate using the WT-Ovation FFPE RNA Amplification System V2. Sense target was generated with the WT-Ovation Exon module and fragmented and labeled with the FL-Ovation cDNA Biotin module. Results showed that BAG3, a protein co-chaperone, is regulated by HSF1. BAG3 expression significantly attenuates HNE-mediated apoptosis in colon cancer cells. Silencing HSF1 results in diminished expression of BAG3. The decreased expression of BAG3 in turn leads to diminished levels of Bcl-XL, Mcl-1, and Bcl-2 protein expression, however, the mRNA levels of these genes were not affected by knockdown of HSF1 or BAG3, suggesting that the effect is mediated at the level of protein turnover. BAG3 has also been shown to inhibit the degradation of Hsp70 client proteins, and the authors propose that the cytoprotective mechanism of BAG3 involves the stabilization of the anti-apoptotic Bcl-2 family members in a multi-protein complex with BAG3 and Hsp70. http://www.jbc.org/cgi/reprint/M808656200v1.pdf NuGEN Publication List View a complete list of scientific publications that utilize NuGEN's products. If you have published using NuGEN technology and don't find it in our listing, please contact NuGEN at techserv@nugeninc.com. Upcoming Events
DATE: October 21st TIME: 11:30 AM-1:00 PM PLACE: Room 304 of the Honolulu Convention Center ENTER AND WIN Stop by and visit us at booth 434 and enter to win a Wii NCRI October 4-7, 2009 National Cancer Research Institute Conference Poster session details: Title: Whole transcriptome amplification from single-cell amounts of total RNA or direct cell lysates and analysis by microarrays Poster Session: A Poster Number: 22 Birmingham, UK Featured Customer Spotlight Andrew I. Brooks, PhD, Director of the BRTC at University of Medicine and Dentistry of New Jersey outlines the benefits and features of using the WT-Ovation™ RNA Amplification System when performing real-time qPCR research. 
WT-Ovation™ One-Direct Amplification System Technical Report and Webinar Now you can conduct global, whole-transcriptome expression analysis from a single cell or just a few cells using microarrays or qPCR. The NuGEN WT-Ovation™ One-Direct System lets you go directly from cell lysate to analysis in just one day without inefficient and time-consuming RNA purification. This webinar will introduce you to true, single cell biology and research studies or download the latest One-Direct System Technical Report. View a Pre-recorded Webinar and Explore single cell biology: NuGEN Technologies presents "Whole Transcriptome Amplification From 'Single-Cell' Amounts of Total RNA presented by Leah Turner, PhD, Sr. Scientist Product Development. View Webinar Download Technical Report: WT-Ovation™ One-Direct RNA Amplification System Performance NuGEN User Guide Update NuGEN will be moving towards an electronic format for user guides and quick protocols in an effort to continue to be environmentally conscious as well as provide customers with easy and effective technical tools. Instead of sending these documents with each shipment we will be directing customers to our website to download the latest technical information. A package insert will be shipped with each product shipment to direct you to the latest user guides and quick protocols on-line. Visit www.nugeninc.com/user-guides to find an overview of the user guide updates. WT-Ovation™ Exon Module Technical Report For whole transcriptome expression analysis, the NuGEN WT-Ovation™ Exon Module works in concert with the Ovation family of amplification products to produce Sense Target (ST) cDNA for use with the GeneChip® Exon ST 1.0 and Gene ST 1.0 expression arrays. Download our latest Technical Report to see how the Exon Module facilitates the study of gene expression and alternative splicing in fresh-frozen and FFPE samples. If this message has been forwarded to you, you may join our mailing list. NuGEN Technologies, Inc. 821 Industrial Road, Unit A San Carlos, CA 94070 Telephone: 888-654-6544 or 650-590-3600 www.nugeninc.com |
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