Our Technology
High-fidelity, sensitive and powerful technologies that revolutionize the analysis of the most challenging genomic samples and let you imagine more from less.
SPIA (Single Primer Isothermal Amplification) — NuGEN's proprietary SPIA® technology (Single Primer Isothermal Amplification) is an elegant method for robust isothermal amplification of nucleic acids used in whole genome and whole transcriptome amplification. This simple, high-fidelity approach to amplification enables global genomic analysis of limited and/or compromised biological samples, overcoming the challenges of sample quality and quantity, and enabling researchers to analyze laser captured microdissections, fine-needle aspirates, sorted cells, circulating tumor cells, embryonic structures and other precious clinical samples such as biopsies and formalin-fixed paraffin-embedded (FFPE) samples.
Selective Sequence Priming and Enrichment — through proprietary primer design strategies combined with innovative assay design, NuGEN has developed a highly sophisticated technology for selectively enriching target sequences in the reverse transcription and first strand cDNA synthesis steps. With these strategies, desired sequences can be made into NGS libraries in a streamlined workflow bypassing any column or bead-based sequence enrichment procedure for substantially improved efficiency, throughput, automatability and data quality.
InDA-C (Insert Dependent Adapter Cleavage) — the novel and NuGEN-proprietary InDA-C method employs customized probes during library creation to target specific transcript species for exclusion in RNA-Seq libraries. Unlike methods that use hybridization-mediated pull-down strategies to deplete unwanted RNA species prior to cDNA synthesis, the InDA-C method selectively targets and eliminates unwanted transcripts from finished libraries, avoiding potential off-target mRNA cross-hybridization events that have been demonstrated to introduce unwanted bias. The InDA-C method is currently employed in the Encore Complete Prokaryotic Library Systems to reduce ribosomal RNA transcripts and in the Encore Complete Whole Blood Library Systems to reduce both human globin and ribosomal RNA transcripts.
High Efficiency Library Adaptors — custom modified adaptors together with optimized ligation reaction conditions allow for the efficient construction of libraries using very low amounts of insert DNA (as little as 10 pg) with little or no adaptor dimer formation. High complexity human genomic libraries can be generated with as little as 10 ng fragmented DNA. These adaptors are an integral part of NuGEN's novel NGS library systems.
Selective Adapter Orientation — this strategy enables directional NGS RNA-Seq library generation. The use of novel, directionally-tagged adaptors during adaptor ligation allows only a single orientation of transcript to continue to library enrichment and cluster formation, resulting in sense-strand sequence generation during the forward read.
Methyl-Track Adaptors — used to generate sequencing libraries compatible with bisulfite conversion. The method incorporates the same technology used in the Ovation® Ultralow Library System that prevents adaptor dimer artifacts and therefore allows successful library generation from as little as 10 ng of human whole genome sample without any gel purification steps. In addition, resulting libraries are directional where Read 1 sequences correspond to the bisulfite converted original genomic strand. The technology significantly reduces the input amount of starting material and greatly simplifies the workflow of targeted bisulfite sequencing and analysis. Reduced input requirements enable analysis of a broader range of sample types down to a few cells.
Single Primer Enrichment Technology — a novel approach developed by NuGEN for targeted DNA resequencing that uses as little as 10ng of genomic DNA input to resequence regions of up to 8Mb without the need for any pre-hybridization amplification of target DNA. The method uses a single targeting probe hybridizing to a gDNA target and then extending that probe through the region of interest. This approach eliminates the difficulty of designing specific PCR primer pairs and maintains high specificity of recovered target sequences in the final library. Assay times are less than eight hours from gDNA to library.
Digital MicroFluidics — describes the micromanipulation of discrete, nanoliter droplets through the application of an electrical voltage to alter the hydrophobicity of the surface on the Mondrian™ SP+ Cartridge. Unit-sized packets of fluid can be transported, mixed, separated and reacted in a way that completely automate complex procedures within the low cost, disposable cartridge. This innovative technology enables reliable, automated liquid handling without the use of tubes, valves, channels or pumps to deliver enormous productivity benefits across a range of life science applications.