Interested in expression profiling of FFPE-derived RNA samples?

Dear Researcher,

Thank you for your interest in the WT-Ovation™ FFPE System. This whole transcriptome amplification solution enables gene expression profiling of small and degraded FFPE-derived RNA using a simple, sensitive, reproducible, and easy to automate process.

Please complete the following brief questionnaire to help us better address your challenges and to provide you the enabling solutions you have come to expect from NuGEN.

Best Regards,
The NuGEN Team

 

1. Have you wanted to perform gene expression studies on FFPE-derived RNA, if so, what would be the significance of success in those studies to you?

2. What analytical platforms would you like to use for your gene expression studies? For example, qPCR, Affymetrix GeneChip®, other?

3. What type of data would convince you to use a few target prep method for your FFPE samples for RNA expression studies?

4. How many FFPE samples would you be interested in processing?

5. What are your FFPE samples' tissue types, age range, and storage conditions?

6. Have you isolated RNA from your FFPE samples, if so what methods or procedures have you used?

7. What did you like and dislike about these purification methods you have tired?

8. Have you ever tried to amplify RNA isolated from FFPE, if so, what methods or procedures have you used?

9. What did you like and dislike about these amplification methods you have tired?

10. What QC methods or metrics do you use to analyze quality and quantity of your FFPE RNA?

11. If you have performed FFPE RNA gene expression profiling on GeneChip® arrays what have been typical percent present calls?

12. Would you be interested in automating FFPE sample processing?

13. Do you currently have FFPE samples under study?

14. Do you anticipate an increase or decrease in your FFPE sample processing

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Thank you very much for completing this Questionnaire.