Encore™ NGS Multiplex System IB
The Encore NGS Library Systems provides a simple, fast, and scalable solution for producing DNA libraries used in a wide range of sequencing applications including RNA-Seq, Digital Gene Expression (DGE), genomic DNA/exome sequencing, amplicon sequencing, ChIP-Seq and more. The Encore NGS Multiplex System IB includes 8 indexed adaptors and together with the original Encore NGS Multiplex System enables creation of up to 16 sequencing libraries with unique index tags.
As shown in Figure 1, the streamlined workflow consists of four main steps: (1) Fragmentation of either genomic DNA or double-stranded cDNA to produce fragments with a tight size distribution; (2) End repair to generate blunt ends; (3) Adaptor ligation for multiplexing or no multiplexing; and (4) PCR amplification to produce the final library. The entire workflow including fragmentation can be completed in as little as three hours, and yields DNA libraries ready for cluster formation and either single read or paired-end sequencing.
The Encore NGS Library Systems have been designed for seamless integration with the Ovation RNA-Seq System, Ovation RNA-Seq FFPE System, and Ovation 3'-DGE System to enable a complete end-to-end solution for transcriptome library construction using unfractionated total RNA samples in 9-10 hours. NuGEN's library products also integrate with the Ovation WGA FFPE System for DNA sequencing from this challenging class of samples.
Table 1 shows a clear segregation of libraries using the index tags, and even distribution of reads derived from libraries containing each index. There is no preferential cluster formation or sequencing from any of the indexed libraries. The theoretical distribution of indexed Reads for a 16-plex run is 6.25%.
Together, the portfolio of integrated NGS products from NuGEN provides a complete, flexible and robust solution to all your NGS sample preparation needs.
