Encore™ ds-DNA Module
The double-stranded DNA generation process used in the Encore ds-DNA Module utilizes a robust enzymatic methodology to generate blunt-ended DNA suitable for generation of NGS sequencing libraries. The Encore ds-DNA Module is validated for use with amplified SPIA Product generated using the Ovation WGA FFPE System (Part No. 6200). The double-stranded DNA integrates directly with the Encore NGS Library System I (Part No. 300) and the Encore NGS Multiplex System I (Part No. 301) for construction of NGS libraries used with the Illumina sequencing platforms. Libraries may also be constructed for other NGS platforms and library construction methods that utilize double-stranded DNA as input.
Quality Sequencing Results to Enable a Range of NGS Applications: In order to make an accurate determination of genomic variations, amplification error rates and bias must be minimized. To address these variables, S. cerevisiae DNA samples were prepared using the Ovation WGA FFPE System together with the Encore ds-DNA Module to make double-stranded DNA for input into the Encore NGS Multiplex System I. Libraries from duplicate amplifications (WGA1 and WGA2) as well as unamplified gDNA were sequenced on the Illumina Genome AnalyzerIIx (results shown above).
The sequencing performance metrics for all samples demonstrated a high percentage of aligned reads and low error rates. Importantly, both the error rates and percent chimeras (large sequences that are replicated during sample preparation) were very similar in both WGA samples and unamplified material.
