Encore™ 384 Multiplex System
The Encore™ 384 Multiplex System is a commercial solution for deep multiplexing of NGS samples that expands NuGEN's current portfolio of Next Gen Sequencing library kits. The Encore 384 Multiplex System consists of a refined set of 384 molecularly "barcoded" library adaptors that enable deep multiplexing of sequencing samples within a library preparation system that is compatible with high-throughput automation.
Building on previous versions, three key considerations were addressed in the library adaptor design (download the white paper). The codes are "unambiguous," meaning each is separated from all others by at least three discrete base pair differences, alleviating mis-assignment to the wrong sample, and avoiding errors introduced by micro-deletions of one or two nucleotides from the 3' end of the adaptor molecules. Additionally, the codes are situated in-line with the sequencing reads of sample inserts, making the NuGEN multiplexing strategy compatible with simple and economical single-read sequencing across leading NGS platforms. Finally, the barcodes are precisely balanced with all four bases at all eight positions within the codes, enabling accurate calibration of base calls with current NGS base calling software.
To validate the performance of our Encore 384 Multiplex System, we created 96 coded libraries with Escherichia coli (MG1655) genomic DNA and 96 coded libraries with human genomic DNA. Following PCR amplification of each library individually, these 192 libraries were pooled and sequenced as one sample on a single lane of an Illumina GAIIx sequencing run.
The Encore 384 Multiplex System is compatible with high-throughput automation and has been fully automated on the Sciclone® NGS Workstation from Caliper Life Sciences as part of an on-going collaboration between NuGEN and Caliper to provide automation of sample processing for a wide range of samples and applications.
Ordering Information:
| Part No. | Product Name |
|---|---|
| Next-Generation Sequencing Library Kit Products | |
| 0315 | Encore 384 Multiplex System |
| 300 | Encore NGS Library System I |
| 301 | Encore NGS Multiplex System IA |
| 302 | Encore NGS Multiplex System IB |
| 3'-DGE Products | |
| 7200 | Ovation® 3'-DGE System |
| RNA-Seq Products | |
| 7102 | Ovation RNA-Seq System V2 |
| 7150 | Ovation RNA-Seq FFPE System |
| 9030 | Ovation Prokaryotic RNA-Seq System |
