Ovation Pico WTA System was previously named WT-Ovation™ Pico RNA Amplification System
Same great kit with NO changes to the product.
The Ovation Pico and PicoSL WTA Systems provide a sensitive, rapid, and simple method for preparing amplified cDNA from a range of 500 picograms to 50 nanograms of total RNA in less than five hours. These two products are specifically designed for applications with very low RNA input requirements for sensitive detection of transcripts over a wide range of abundance. Amplification is initiated both at the poly(A) tail and randomly throughout the whole transcript for complete coverage and to enable analysis of non-poly(A) transcripts as well as degraded RNA samples.
After amplification, the cDNA product can be used directly for downstream qPCR analysis. Alternatively, to use the amplified product for gene expression profiling on microarrays, simply use either the Encore™ Biotin Module, or follow a validated NuGEN protocol to fragment and label the target, ready for hybridization to the arrays.
With the same input of total RNA and a similar procedure, the Ovation Pico and PicoSL WTA Systems are tailored to produce distinct levels of output meeting varying downstream analysis requirements. The Ovation Pico WTA System generates 6-10 µg of amplified cDNA whereas the Ovation PicoSL WTA System is optimized for lower output at 2-4 µg.
The choice of which product to use should be based on the type of expression microarray used, the number of qPCR markers, as well as any other study design considerations. In general, it is recommended to use the Ovation Pico WTA System for Affymetrix GeneChip® Arrays and the Ovation PicoSL WTA System for microarrays requiring less cDNA target including the Illumina Whole Genome Expression BeadChips or Agilent Dual-Mode Gene Expression Arrays.
Universal Human RNA (UHR) from Stratagene was amplified in quadruplets (2 ng or 500 pg of total RNA) using the Ovation Pico and PicoSL WTA Systems. The anticipated yields were produced suitable for different downstream analysis requirements.
Colon total RNA was amplified in triplicate with a wide range of input using the Ovation Pico WTA System. Five qPCR assays were designed and performed for three transcripts and Ct values were normalized to the PGK transcript. qPCR results are shown with Delta Ct plotted against the RNA input and demonstrate that the Ct values from each set of transcripts remain consistent across a wide range (100 pg to 50 ng) of total RNA input for all interrogated transcripts, even at RNA input levels far below the recommended range.
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