Ovation® RNA-Seq System
The Ovation RNA-Seq System provides a fast and simple method for preparing amplified cDNA from total RNA for RNA-Seq applications (transcriptome sequencing). Amplification is initiated at the 3' end as well as randomly throughout the whole transcriptome in the sample. This feature makes the Ovation RNA-Seq System ideal for amplification prior to Next Generation Sequencing, as it allows amplification of both mRNA and non-polyadenylated transcripts. The double-stranded cDNA product of the Ovation RNA-Seq System is optimized for the generation of sequencing libraries for Next Generation Sequencing platforms.
The Ovation RNA-Seq System is powered by Ribo-SPIA technology, a rapid, simple and sensitive RNA amplification process developed by NuGEN. Using Ribo-SPIA technology and starting with as little as 500 pg total RNA, microgram quantities of cDNA can be prepared in approximately 6 hours.
The Ovation RNA-Seq System (Cat. # 7100-08) provides optimized reagent mixes and a protocol to process 8 total RNA samples.
Comparison of Ovation RNA-Seq and qPCR: Differential expression data was generated for the MAQC A (Human Universal Reference) and MAQC B (Human Brain Reference) by both RNA-Seq and qPCR. RNA-Seq data from 10 ng of total RNA is plotted on X-axis, qPCR data plaotted Y-axis. 659 TaqMan probes that uniquely map to the refSeq annotations used in the RPKM calculations are represented. The qPCR results were originally reported in Shi, L. et al. Nature Biotechnology (2006) 24(9): 1151-1161.
Inter-user Reproducibility: Scatterplot of the log2 RPKM values for two Human Reference Brain samples (designated as S1 and S2) prepared using 10 ng of total RNA and amplified by two independent operators with the Ovation RNA-Seq System. RPKM stands for Reads Per Kilobase of exon model per Million mapped reads. The RPKM measure of read density reflects the molar concentration of a transcript in the starting sample by normalizing for RNA length and for the total read number in the measurement. Sequencing results were obtained using the Illumina Genome Analyzer IIx platform.
Ordering Information
