Ovation® RNA Amplification System V2
The Ovation RNA Amplification System V2 provides a rapid, simple, and automation-friendly approach for 3'-focused amplification of total RNA in under four hours, and yields between four and seven µg of amplified antisense ss-cDNA for qPCR or microarray analysis.
The amplified cDNA can be fragmented and labeled in under two hours for Affymetrix GeneChip® 3’ expression array analysis using NuGEN’s FL-Ovation™ cDNA Biotin Module V2.
For analysis on Agilent arrays, the amplified cDNA can be fragmented and labeled in the same day using NuGEN’s FL-Ovation™ cDNA Fluorescent Module.
The Ovation™ RNA Amplification System V2 provides optimized reagent mixes and a protocol to process 12 or 60 RNA samples (cat.# 3100-12, 3100-60, 3100-A01 [automation]).
High level of reproducibility with as little as 5 ng of total RNA: Two independent amplifications of 5 ng HeLa RNA using the Ovation™ RNA Amplification System V2, processed with the FL-Ovation™ cDNA Biotin Module V2, and analyzed on Affymetrix HG-U133A 2.0 GeneChip® array, show a high R2 signal correlation of 0.994 with a call concordance of 93.2%.
